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Steven Salzberg
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Steven Lloyd Salzberg is an American computational biologist and computer scientist who is a Bloomberg Distinguished Professor of Biomedical Engineering, Computer Science, and Biostatistics at Johns Hopkins University, where he is also Director of the Center for Computational Biology.
Steven Salzberg at the Biological Data Science conference (CSHL) in 2018
RNA-Seq
Videos
RNA-Seq is a technique that uses next-generation sequencing to reveal the presence and quantity of RNA molecules in a biological sample, providing a snapshot of gene expression in the sample, also known as transcriptome.
Examples of alternative RNA splicing modes. Exons are represented as blue and yellow blocks, spliced introns as horizontal black lines connecting two
Image: RNA Seq Pics 1
Image: RNA Seq Pics 1
Typical single-cell RNA-Seq workflow. Single cells are isolated from a sample into either wells or droplets, cDNA libraries are generated and amplifie
Typical single-cell RNA-Seq workflow. Single cells are isolated from a sample into either wells or droplets, cDNA libraries are generated and amplified, libraries are sequenced, and expression matrices are generated for downstream analyses like cell type identification.