Subiaco is a comune (municipality) in the Metropolitan City of Rome Capital, in the Italian region of Latium, 40 kilometres (25 mi) from Tivoli alongside the River Aniene. It is a tourist and religious resort because of its sacred grotto, in the medieval St. Benedict's Abbey, and its Abbey of Santa Scolastica. It is one of I Borghi più belli d'Italia. The first books to be printed in Italy were produced here in the late 15th century.

The Aniene, formerly known as the Teverone, is a 99-kilometer (62 mi) river in Lazio, Italy. It originates in the Apennines at Trevi nel Lazio and flows westward past Subiaco, Vicovaro, and Tivoli to join the Tiber in northern Rome. It formed the principal valley east of ancient Rome and became an important water source as the city's population expanded. The falls at Tivoli were noted for their beauty. Historic bridges across the river include the Ponte Nomentano, Ponte Mammolo, Ponte Salario, and Ponte di San Francesco, all of which were originally fortified with towers.