The Tampa Theatre is a historic U.S. theater and city landmark in Downtown Tampa, Florida. Designed as an atmospheric theatre-style movie palace by architect John Eberson, it opened on October 15, 1926. The theatre features a wide range of independent, foreign, and documentary films. It is Tampa's only non-profit movie palace, and operating costs are supported by its members, donors, and corporate sponsors, as well as by ticket and concessions sales. It has often been used as a backdrop for movies, music videos, and local programming.

John Adolph Emil Eberson was an Austrian-American architect best known for the development and promotion of movie palace designs in the atmospheric theatre style. He designed over 500 theatres in his lifetime, earning the nickname "Opera House John". His most notable surviving theatres in the United States include the Tampa Theatre (1926), Palace Theatre Marion (1928), Palace Theatre Louisville (1928), Majestic Theatre (1929), Akron Civic Theatre (1929), the Paramount Theatre (1929), the State Theater 1927, and the Lewis J. Warner Memorial Theater (1932) at Worcester Academy in Worcester, Massachusetts. Remaining international examples in the atmospheric style include both the Capitol Theatre (1928) and State Theatre (1929) in Sydney, Australia, The Forum and Le Grand Rex.