The women's singles was an event on the Tennis at the 1900 Summer Olympics program in Paris. It was held from 6 to 11 July at the Île de Puteaux. There were 6 competitors from 4 nations. The event was won by Charlotte Cooper of Great Britain. France's Hélène Prévost was the silver medalist, while American Marion Jones Farquhar and Hedwiga Rosenbaumová of Bohemia are credited with bronze medals. The event made Cooper the first female individual Olympic champion.

Charlotte "Chattie" Cooper Sterry was an English female tennis player who won five singles titles at the Wimbledon Championships and in 1900 became Olympic champion. In winning in Paris on 11 July 1900, she became the first female Olympic tennis champion as well as the first individual female Olympic champion.