Puerto Rico's Monica Puig defeated Germany's Angelique Kerber in the final, 6–4, 4–6, 6–1 to win the gold medal in Women's Singles tennis at the 2016 Summer Olympics. It was Puerto Rico's first Olympic gold medal, and Puig became the first unseeded female player to win the Olympic tennis gold medal. In the bronze-medal match, the Czech Republic's Petra Kvitová defeated the United States' Madison Keys 7–5, 2–6, 6–2. It was Germany's first medal in women's singles since 1992 and the Czech Republic's first since 1996.

Puerto Rico competed at the 2016 Summer Olympics in Rio de Janeiro, Brazil, from 5 to 21 August 2016. This was the nation's eighteenth consecutive appearance at the Summer Olympics.