The Third Mithridatic War, the last and longest of the three Mithridatic Wars, was fought between Mithridates VI of Pontus and the Roman Republic. Both sides were joined by a great number of allies, dragging the entire east of the Mediterranean and large parts of Asia into the war. The conflict ended in defeat for Mithridates; it ended the Pontic Kingdom and the Seleucid Empire, and also resulted in the Kingdom of Armenia becoming an allied client state of Rome.

Mithridates or Mithradates VI Eupator was the ruler of the Kingdom of Pontus in northern Anatolia from 120 to 63 BC, and one of the Roman Republic's most formidable and determined opponents. He was an effective, ambitious and ruthless ruler who sought to dominate Asia Minor and the Black Sea region, waging several hard-fought but ultimately unsuccessful wars to break Roman dominion over Asia and the Hellenic world. He has been called the greatest ruler of the Kingdom of Pontus. He cultivated an immunity to poisons by regularly ingesting sub-lethal doses; this practice, now called mithridatism, is named after him. After his death, he became known as Mithridates the Great.