The Timucua were a Native American people who lived in Northeast and North Central Florida and southeast Georgia. They were the largest indigenous group in that area and consisted of about 35 chiefdoms, many leading thousands of people. The various groups of Timucua spoke several dialects of the Timucua language. At the time of European contact, Timucuan speakers occupied about 19,200 square miles (50,000 km2) in the present-day states of Florida and Georgia, with an estimated population of 200,000. Milanich notes that the population density calculated from those figures, 10.4 per square mile (4.0/km2) is close to the population densities calculated by other authors for the Bahamas and for Hispaniola at the time of first European contact. The territory occupied by Timucua speakers stretched from the Altamaha River and Cumberland Island in present-day Georgia as far south as Lake George in central Florida, and from the Atlantic Ocean west to the Aucilla River in the Florida Panhandle, though it reached the Gulf of Mexico at no more than a couple of points.

The Agua Dulce or Agua Fresca (Freshwater) were a Timucua people of northeastern Florida. They lived in the St. Johns River watershed north of Lake George, and spoke a dialect of the Timucua language also known as Agua Dulce.