The Tissington Trail is a bridleway, footpath and cycleway in Derbyshire, England, along part of the trackbed of the former railway line connecting Ashbourne to Buxton. It takes its name from the village of Tissington, which it skirts. Opened in 1971, and now a part of the National Cycle Network, it stretches for 13 miles (21 km) from Parsley Hay (53.1706°N 1.7828°W) in the north to Ashbourne (53.0196°N 1.7397°W) in the south.

The Ashbourne line was a 33+1⁄2 mi (53.9 km) railway from Buxton via Ashbourne to Uttoxeter. It was built by the London and North Western Railway using a section of the Cromford and High Peak Railway (C&HPR) and it joined the North Staffordshire Railway at Ashbourne, proceeding to Uttoxeter with a junction onto the main line at Rocester.