Transport in Panama covers a vast network of routes predominantly traversed by cargo trucks and buses. The country's railway system, known as the Panama Canal Railway, facilitates the transportation of both passengers and goods. With a total of 15,137 km of roads, its four expressways—Corredor Sur, Corredor Norte, Autopista La Chorrera, and Colón Panama—are the arteries that link various parts of the country. Tocumen International Airport (PTY) is the key hub for international travel, ranking among the largest and most important airports in Latin America. Additionally, the renowned Panama Canal links the mere 60 km that separate the Pacific Ocean and Atlantic Ocean, offering an alternative route for maritime cargo transportation, avoiding the need to sail around South America.

The Panama Canal Railway is a railway line linking the Atlantic Ocean to the Pacific Ocean in Central America. The route stretches 47.6 miles (76.6 km) across the Isthmus of Panama from Colón (Atlantic) to Balboa. Because of the difficult physical conditions of the route and state of technology, the construction was renowned as an international engineering achievement, one that cost US$8 million and the lives of an estimated 5,000 to 10,000 workers. Opened in 1855, the railway preceded the Panama Canal by half a century; the railway was vital in assisting the construction of the canal in the early 1900s. With the opening of the canal, the railroad's route was changed as a result of the creation of Gatun Lake, which flooded part of the original route. Following World War II, the railroad's importance declined and much of it fell into a state of neglect until 1998, when a project to rebuild the railroad to haul intermodal traffic began; the new railroad opened in 2001.