The Tribe of Joseph is one of the Tribes of Israel in biblical tradition. Since the tribes of Ephraim and Manasseh together traditionally constituted the "tribe of Joseph", it was often not listed as one of the tribes, in favour of Ephraim and Manasseh being listed in its place; consequently it was often termed the House of Joseph, to avoid the use of the term tribe. Even though Ephraim and Manasseh were Joseph’s sons, they were considered heads of two of the twelve tribes of Israel because Joseph’s father, Jacob, adopted Ephraim and Manasseh as his own sons.

The Twelve Tribes of Israel are, according to Hebrew scriptures, the descendants of the biblical patriarch Jacob, who collectively form the Israelite nation. The tribes were through his twelve sons through his wives, Leah and Rachel, and his concubines, Bilhah and Zilpah. In modern scholarship, there is skepticism as to whether there ever were twelve Israelite tribes, with the use of the number 12 thought more likely to signify a symbolic tradition as part of a national founding myth, although some scholars disagree with this view.