Troutville is a town in Botetourt County, Virginia, United States. The population was 458 at the 2020 census. The town is located along the U.S. Route 11 between Cloverdale and Buchanan. It is part of the Roanoke Metropolitan Statistical Area.

Botetourt County is a US county that lies in the Roanoke Region of Virginia. Located in the mountainous portion of the state, the county is bordered by two major ranges, the Blue Ridge Mountains and the Appalachian Mountains.