Tusculum is a city in Greene County, Tennessee, United States. The population was 3,298 at the 2020 census. It is the site of Tusculum University, the oldest university in Tennessee and the 28th oldest in the United States. Tusculum is a suburb of nearby Greeneville. The population of both Greeneville and Tusculum combined was approximately 18,777 at the 2020 census.

Greene County is a county located on the eastern border of the U.S. state of Tennessee. As of the 2020 census, the population was 70,152. Its county seat is Greeneville. Greene County comprises the Greeneville, TN Micropolitan Statistical Area.