TwinBee (ツインビー) is a video game series composed primarily of cartoon-themed vertical-scrolling shoot-'em-up games produced by Konami that were released primarily in Japan. The series originated as a coin-operated video game simply titled TwinBee in 1985, which was followed by several home versions and sequels. The character designs of almost every game in the series since Detana!! TwinBee in 1991 were provided by Japanese animator Shuzilow HA, who also planned and supervised most of the subsequent installments in the TwinBee series. The series also inspired a radio drama adaptation that lasted three seasons in Japan, as well as an anime adaptation.

Shoot 'em ups are a sub-genre of action games. There is no consensus as to which design elements compose a shoot 'em up; some restrict the definition to games featuring spacecraft and certain types of character movement, while others allow a broader definition including characters on foot and a variety of perspectives.