In the Book of Revelation, the two witnesses are two prophets who are mentioned in Revelation 11:1-14. Some Christians interpret this as two people, two groups of people, or two concepts. Some believe they are Enoch and Elijah, as in the Gospel of Nicodemus, since they are the only two that did not see death as required by the Scriptures, while others believe them to be Moses and Elijah because they appeared during the transfiguration of Jesus, or because Enoch was not Abraham's descendant. They have the power to shut the heavens (Elijah) and turn water into blood (Moses).

Heaven's Gate was an American new religious movement known primarily for the mass suicides committed by its members in 1997. Commonly designated a cult, it was founded in 1974 and led by Bonnie Nettles (1927–1985) and Marshall Applewhite (1931–1997), known within the movement as Ti and Do, respectively. Nettles and Applewhite first met in 1972 and went on a journey of spiritual discovery, identifying themselves as the two witnesses of Revelation, attracting a following of several hundred people in the mid-1970s. In 1976, a core group of a few dozen members stopped recruiting and instituted a monastic lifestyle.