U.S. Highway 25 (US 25) is a United States Numbered Highway that travels from Brunswick, Georgia, to the Kentucky–Ohio state line, where Covington, Kentucky, meets Cincinnati, Ohio, at the Ohio River. In the U.S. state of Georgia, US 25 is as a 190.0-mile-long (305.8 km) highway that travels south to north in the eastern part of the state, near the Atlantic Ocean, serving Statesboro and the Brunswick and Augusta metropolitan areas on its path from Brunswick to South Carolina at the Savannah River. Its routing travels through portions of Glynn, Wayne, Long, Tattnall, Evans, Bulloch, Jenkins, Burke, and Richmond counties.

Wayne County is a county located in the southeastern part of the U.S. state of Georgia. As of the 2020 census, the population was 30,144. The county seat is Jesup.