U.S. Highway 29 (US 29) in the state of Florida is the westernmost north–south United States Numbered Highway in the state. It runs 43.6 miles (70.2 km) from downtown Pensacola north to the Alabama state line entirely within Escambia County. US 29 runs as a four-lane highway through much of the Florida Panhandle, becoming six lanes through and near several towns. The highway's hidden state road designation is entirely State Road 95 (SR 95).

Escambia County is the westernmost and oldest county in the U.S. state of Florida. It is in the state's northwestern corner. At the 2020 census, the population was 321,905. Its county seat and largest city is Pensacola. Escambia County is included within the Pensacola—Ferry Pass—Brent, Florida Metropolitan Statistical Area. The county population has continued to increase as the suburbs of Pensacola have developed.