U.S. Route 331 is a spur of U.S. Route 31. It currently runs for 150 miles (240 km) from Santa Rosa Beach, Florida east of Fort Walton Beach at US 98 to Montgomery, Alabama at US 80 and US 82. Unlike US 131, US 231, and US 431, US 331 never intersects with its "parent" route, US 31; however, the two routes do come within 4 miles (6.4 km) of each other in Montgomery.

U.S. Route 80 (US 80) is a major U.S. Highway in the American state of Alabama. The Alabama Department of Transportation internally designates the majority of US 80 throughout the state as State Route 8 (SR 8), save for parts of the route throughout Selma and near the Mississippi border. Serving as the main east to west highway through Alabama's Black Belt region, US 80 became well known as the main route for the 1965 Selma to Montgomery marches; it was the route along which the Civil Rights demonstrators walked, from Selma to Alabama, and the Edmund Pettus Bridge in Selma was the site of Bloody Sunday. The highway was also once a major transcontinental highway reaching from Tybee Island, Georgia, to San Diego, California, but has since been truncated to Dallas, Texas because it was largely replaced by the Interstate Highway System.