U.S. Highway 34 (US 34) is a United States Highway that runs across the southern third of Iowa. It begins on a bridge over the Missouri River west of Glenwood and travels east where it meets Interstate 29 (I-29) and US 275. Through southwestern Iowa, the highway is, for the most part, a two-lane rural road with at-grade intersections; there are interchanges with US 59 near Emerson and US 71 near Stanton and Villisca. At Osceola, the highway intersects I-35 and US 69. Just east of Ottumwa, where the road meets US 63, the road joins the four-lane Iowa 163 for the remainder of its trek through the state. At Mount Pleasant, it overlaps US 218 and Iowa 27, the Avenue of the Saints Highway. From there, the road heads to the southeast where it crosses the Mississippi River on the Great River Bridge at Burlington.

In the US state of Iowa, Interstate 29 (I-29) is a north–south Interstate Highway which closely parallels the Missouri River. I-29 enters Iowa from Missouri near Hamburg and heads to the north-northwest through the Omaha–Council Bluffs and the Sioux City areas. It exits the state by crossing the Big Sioux River into South Dakota. For its entire distance through the state, it runs on the flat land between the Missouri River and the Loess Hills.