The United States Fish and Wildlife Service is a U.S. federal government agency within the U.S. Department of the Interior which oversees the management of fish, wildlife, and natural habitats in the United States. The mission of the agency is "working with others to conserve, protect, and enhance fish, wildlife, plants and their habitats for the continuing benefit of the American people."

The United States Department of the Interior (DOI) is an executive department of the U.S. federal government responsible for the management and conservation of most federal lands and natural resources. It also administers programs relating to Native Americans, Alaska Natives, Native Hawaiians, territorial affairs, and insular areas of the United States, as well as programs related to historic preservation. About 75% of federal public land is managed by the department, with most of the remainder managed by the Department of Agriculture's Forest Service. The department was created on March 3, 1849. It is headquartered at the Main Interior Building, located at 1849 C Street NW in Washington, D.C.