The University of Mount Olive is a private university in Mount Olive, North Carolina. Chartered in 1951, the university is sponsored by the Original Free Will Baptist Convention and accredited by the Southern Association of Colleges and Schools Commission on Colleges. A member of the NCAA Division II Conference Carolinas, its sports teams compete as the Mount Olive Trojans.

Mount Olive is a town in Duplin and Wayne counties in the U.S. state of North Carolina. The population was 4,589 at the 2010 census. It is included in the Goldsboro, North Carolina Metropolitan Statistical Area. The town is home to the Mt. Olive Pickle Company and the University of Mount Olive.