The University of Toulouse is a community of universities and establishments (ComUE) based in Toulouse, France. Originally it was established in 1229, making it one of the earliest universities to emerge in Europe. Suppressed during the French Revolution in 1793, it was refounded in 1896 as part of the reorganization of higher education. It was finally abolished in 1969, giving birth to the three current universities: Toulouse 1 Capitole University, University of Toulouse-Jean Jaurès and Toulouse III - Paul Sabatier University. The ComUE in the Toulouse region was known as Federal University of Toulouse Midi-Pyrénées. On January 1, 2023, the university was renamed as the University of Toulouse.

Toulouse is the prefecture of the French department of Haute-Garonne and of the larger region of Occitania. The city is on the banks of the River Garonne, 150 kilometres from the Mediterranean Sea, 230 km (143 mi) from the Atlantic Ocean and 680 km (420 mi) from Paris. It is the fourth-largest city in France after Paris, Marseille and Lyon, with 504,078 inhabitants within its municipal boundaries (2021); its metropolitan area has a population of 1.5 million inhabitants (2021). Toulouse is the central city of one of the 22 metropolitan councils of France. Between the 2014 and 2020 censuses, its metropolitan area was the third fastest growing among metropolitan areas larger than 500,000 inhabitants in France.