Venezia Mestre railway station is a junction station in the comune of Venice, Italy. It is located within the mainland frazione of Mestre, and is classified by its owner, Rete Ferroviaria Italiana, as a gold category station.

The Milan–Venice railway line is one of the most important railway lines in Italy. It connects the major city of Milan, in Lombardy, with the Adriatic Sea at Venice, in Veneto. The line is state-owned and operated by the state rail infrastructure company, Rete Ferroviaria Italiana that classifies it as a trunk line. The line is electrified at 3,000 volts DC.