John Wayne Mixson was an American politician and farmer in Florida who served as the 12th lieutenant governor of Florida from 1979 to 1987, and as the 39th governor of Florida for three days in January 1987. Mixson served in the Florida House of Representatives from 1967 to 1978 prior to being elected as lieutenant governor. He was a lifelong conservative Democrat, and though he served in the Florida legislature and as Florida's lieutenant governor as a member of that party, he supported a mix of Democratic and Republican candidates for various state and national offices after retiring from elected office.

The lieutenant governor of Florida is a statewide constitutional office in the executive branch of the U.S. state of Florida. It is the second highest-ranking official in the state government. The lieutenant governor is elected on a ticket with the governor for a four-year term.