The Weeden Island cultures are a group of related archaeological cultures that existed during the Late Woodland period of the North American Southeast. The name for this group of cultures was derived from the Weedon Island site in Old Tampa Bay in Pinellas County.

In the classification of archaeological cultures of North America, the Woodland period of North American pre-Columbian cultures spanned a period from roughly 1000 BCE to European contact in the eastern part of North America, with some archaeologists distinguishing the Mississippian period, from 1000 CE to European contact as a separate period. The term "Woodland Period" was introduced in the 1930s as a generic term for prehistoric sites falling between the Archaic hunter-gatherers and the agriculturalist Mississippian cultures. The Eastern Woodlands cultural region covers what is now eastern Canada south of the Subarctic region, the Eastern United States, along to the Gulf of Mexico.