The Western Mustangs are the athletic teams that represent Western University in London, Ontario, Canada. The school's athletic program supports 46 varsity teams. Their mascot is a Mustang named J.W. and the school colours are purple and white. The university's varsity teams compete in the Ontario University Athletics conference and the national U Sports organization. Western University offers 21 varsity sports for men and 19 for women which compete in the OUA conference. The university also offers cheerleading, women's ringette, women's softball, table tennis and ultimate frisbee, which compete outside the OUA conference, in sport-specific conferences and divisions.

The U Sports Women's Volleyball Championship is a Canadian university volleyball tournament conducted by U Sports, and determines the women's national champion. The tournament involves the champions from each of Canada's four regional sports conferences. The championship trophy, first awarded in 1977, features a two-wheeled oxcart, symbolizing the pioneer era on the Red River in Manitoba. The 2024 champions are the UBC Thunderbirds who have also won the most championships with a total of 14, including six in a row from 2008 to 2013.