Wheeling Island is the most populated island in the Ohio River. It lies within the city of Wheeling in Ohio County, West Virginia, in the United States. The 2000 census showed a resident population of 3,142 people on the island, which has a land area of 1.514 square kilometres. Neville Island, Pennsylvania, is larger but has fewer people (1,232).

The Ohio River is a 981-mile-long (1,579 km) river in the United States. It is located at the boundary of the Midwestern and Southern United States, flowing in a southwesterly direction from western Pennsylvania to its mouth on the Mississippi River at the southern tip of Illinois. It is the third largest river by discharge volume in the United States and the largest tributary by volume of the north-south flowing Mississippi River, which divides the eastern from western United States. It is also the 6th oldest river on the North American continent. The river flows through or along the border of six states, and its drainage basin includes parts of 14 states. Through its largest tributary, the Tennessee River, the basin includes several states of the southeastern U.S. It is the source of drinking water for five million people.