Wisconsin's 32nd Senate district is one of 33 districts in the Wisconsin Senate. Located in western Wisconsin, the district comprises all of La Crosse County and nearly all of Vernon County, along with parts of southwest Monroe County and southeast Trempealeau County. It includes the cities of La Crosse, Onalaska, Sparta, and Viroqua.

The Wisconsin Senate is the upper house of the Wisconsin State Legislature. Together with the larger Wisconsin State Assembly they constitute the legislative branch of the state of Wisconsin. The powers of the Wisconsin Senate are modeled after those of the U.S. Senate.