The 46th Assembly District of Wisconsin is one of 99 districts in the Wisconsin State Assembly. Located in southern Wisconsin, the district comprises most of the west half of Jefferson County and parts of eastern Dane County. It includes the cities of Fort Atkinson, Lake Mills, and Waterloo, and the villages of Cambridge, Deerfield, and Rockdale, along with nearly all of the village of Cottage Grove and part of the village of McFarland. The district also contains Aztalan State Park and the Goose Lake Wildlife Area. The district is represented by Democrat Melissa Ratcliff, since January 2023.

The Wisconsin State Assembly is the lower house of the Wisconsin Legislature. Together with the smaller Wisconsin Senate, the two constitute the legislative branch of the U.S. state of Wisconsin.