State Trunk Highway 16 is a Wisconsin state highway running from Pewaukee across the state to La Crosse. Much of its route in the state parallels the former mainline of the Milwaukee Road and current mainline of the Canadian Pacific Railway. Interstate 90 (I-90) or I-94 parallels Highway 16 for most of its length in the state. It serves local traffic in nearby cities including La Crosse, Tomah, Wisconsin Dells, Portage, Columbus, Watertown, Oconomowoc and Waukesha. The highway is mainly two-lane surface road or urban multi-lane expressway from La Crosse to Oconomowoc, and it is a freeway east of Oconomowoc.

Interstate 94 (I-94) runs east–west through the western, central, and southeastern portions of the US state of Wisconsin. A total of 348.23 miles (560.42 km) of I-94 lie in the state.