State Trunk Highway 27 is a state highway in the US state of Wisconsin. The highway spans a length of 294 miles (473 km) and is generally two-lane local road with the exception of urban multilane arterials within some cities. Earlier routing of the highway had it reaching Superior along the present US Highway 53 (US 53) and Fennimore along the present US 61 and following Interstate 94 (I-94) between Black River Falls and Osseo.

Interstate 94 (I-94) runs east–west through the western, central, and southeastern portions of the US state of Wisconsin. A total of 348.23 miles (560.42 km) of I-94 lie in the state.