Women's colleges in the Southern United States refers to undergraduate, bachelor's degree–granting institutions, often liberal arts colleges, whose student populations consist exclusively or almost exclusively of women, located in the Southern United States. Many started first as girls' seminaries or academies. Salem College is the oldest female educational institution in the South and Wesleyan College is the first that was established specifically as a college for women, closely followed by Judson College in 1838. Some schools, such as Salem College, offer coeducational courses at the graduate level.

Salem College is a private women's liberal arts college in Winston-Salem, North Carolina. Founded in 1772 as a primary school, it later became an academy and ultimately added the college. It is the oldest female educational establishment that is still a women's college and the oldest women's college in the Southern United States.