The Yangon–Mandalay Expressway is a tolled expressway in Myanmar (Burma) that connects the country's largest city Yangon and second largest city Mandalay. Opened in December 2010, the 587 km (365 mi) expressway has reduced the travel time between Yangon and Mandalay to 7 hours from 13 hours by train and from 16 hours by the old highway. The highway, which does not meet international design, construction and safety standards has seen a spate of accidents since its opening.

Mandalay is the second-largest city in Myanmar, after Yangon. Located on the east bank of the Irrawaddy River, 631 km north of Yangon, the city has a population of 1,225,553.