Zane's Trace is a frontier road constructed under the direction of Col. Ebenezer Zane through the Northwest Territory of the United States, in what is now the state of Ohio. Many portions were based on traditional Native American trails. Constructed during 1796 and 1797, the road ran from Wheeling, Virginia to Maysville, Kentucky, through the portion of the Northwest Territory that eventually became the southeastern quarter of the state of Ohio. It was more than 230 miles (370 km) long and was interrupted by several rivers.

Ebenezer Zane was an American pioneer, soldier, politician, road builder and land speculator. Born in the Colony of Virginia, Zane established a settlement near Fort Henry which became Wheeling, on the Ohio River. He also blazed an early road through the Ohio Country to Limestone known as Zane's Trace.