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Zygote

A zygote is a eukaryotic cell formed by a fertilization event between two gametes. The zygote's genome is a combination of the DNA in each gamete, contains all of the genetic information necessary to form a new individual. In multicellular organisms, the zygote is the earliest developmental stage. In single-celled organisms, the zygote can divide asexually by mitosis to produce identical offspring. German zoologists Oscar and Richard Hertwig made some of the first discoveries on animal zygote formation in the late 19th century. In fungi, the sexual fusion of haploid cells is called karyogamy; the result of karyogamy is the formation of a diploid cell called the zygospore. This cell may enter meiosis or mitosis depending on the life cycle of the species. In plants, the zygote may be polyploid if fertilization occurs between meiotically unreduced gametes. In land plants, the zygote is formed within a chamber called the archegonium. In seedless plants, the archegonium is flask-shaped, with a long hollow neck through which the sperm cell enters.

As the zygote divides and grows, it does so inside the archegonium. Main articles: Development of the human body, Human fertilization In human fertilization, a released ovum and a haploid sperm cell —combine to form a single 2n diploid cell called the zygote. Once the single sperm enters the oocyte, it completes the division of the second meiosis forming a haploid daughter with only 23 chromosomes all of the cytoplasm, the sperm in its own pronucleus; the other product of meiosis is the second polar body with only chromosomes but no ability to replicate or survive. In the fertilized daughter, DNA is replicated in the two separate pronuclei derived from the sperm and ovum, making the zygote's chromosome number temporarily 4n diploid. After 30 hours from the time of fertilization, fusion of the pronuclei and immediate mitotic division produce two 2n diploid daughter cells called blastomeres. Between the stages of fertilization and implantation, the developing human is a preimplantation conceptus.

There is some dispute about whether this conceptus should no longer be referred to as an embryo, but should now be referred to as a proembryo, terminology that traditionally has been used to refer to plant life. Some ethicist and legal scholars make the argument that it is incorrect to call the conceptus an embryo, because it will differentiate into both intraembryonic and extraembryonic tissues, can split to produce multiple embryos. Others have pointed out that so-called extraembryonic tissues are part of the embryo's body that are no longer used after birth. Further, as the embryo splits to form identical twins – leaving the original tissues intact – new embryos are generated, in a process similar to that of cloning an adult human. In the US the National Institutes of Health has determined that the traditional classification of pre-implantation embryo is still correct. After fertilization, the conceptus travels down the oviduct towards the uterus while continuing to divide mitotically without increasing in size, in a process called cleavage.

After four divisions, the conceptus consists of 16 blastomeres, it is known as the morula. Through the processes of compaction, cell division, blastulation, the conceptus takes the form of the blastocyst by the fifth day of development, just as it approaches the site of implantation; when the blastocyst hatches from the zona pellucida, it can implant in the endometrial lining of the uterus and begin the embryonic stage of development. The human zygote has been genetically edited in experiments designed to cure inherited diseases; the formation of a totipotent zygote with the potential to produce a whole organism depends on epigenetic reprogramming. DNA demethylation of the paternal genome in the zygote appears to be an important part of epigenetic reprogramming. In the paternal genome of the mouse, demethylation of DNA at sites of methylated cytosines, is a key process in establishing totipotency. Demethylation involves the processes of base excision repair and other DNA- repair- based mechanisms.

A Chlamydomonas zygote contains chloroplast DNA from both parents. These rare biparental zygotes allowed mapping of chloroplast genes by recombination. In the amoeba, reproduction occurs by cell division of the parent cell: first the nucleus of the parent divides into two and the cell membrane cleaves, becoming two "daughter" Amoebae. Breastfeeding and fertility Proembryo

A Daughter's a Daughter

A Daughter's a Daughter is a novel written by Agatha Christie and first published in the UK by Heinemann on 24 November 1952. Unpublished in the US, it was issued as a paperback by Dell Publishing in September 1963, it was the fifth of six novels. A play written by Christie in the late 1930s, the plot tells of a daughter's opposition to her mother's plan to remarry. Christie tried to interest Peter Saunders the producer of The Mousetrap, in the play in 1950, he suggested amendments to update some of the references, which were now twenty years old, tried the play out at the Theatre Royal, Bath where it opened on 9 July 1956 and ran for one week and eight performances. It was billed under the Westmacott name but the true identity of the author slipped out resulting in good attendance figures. Saunders however felt it would not survive in the West End and Christie didn't pursue the matter further. Following Christie's death, the copyright for the play was owned by her daughter, Rosalind Hicks, unenthusiastic about the play as it was believed that the main character was based on her.

Following Hicks' death in 2004, a new production of the play, starring Jenny Seagrove and Honeysuckle Weeks and produced by Bill Kenwright, was to open in London's West End on 14 December 2009. Kenwright described the play as "brutal and honest" and "It's a good enough play to stand up without the Christie brand. It's quite a tough play, it is a substantial night at the theatre." 1952, Heinemann, 24 November 1952, Hardback, 200 pp 1963, Dell Books, September 1963, Paperback, 191 pp 1972, Arbor House, Hardback, 191 pp 1978, Ulverscroft Large-print Edition, Hardcover, 334 pp ISBN 0-7089-0217-0 1986, Fontana Books, Paperback

Chuck Heberling

Charles Heberling was an American football official in the National Football League and Western Pennsylvania sports administrator. He was the referee for both The Drive, he was an alternate for the officiating crew for Super Bowl XXI. Heberling wore the number 46 for the major part of his NFL career. Upon his inauguration into the Washington & Jefferson College Hall of Fame, his alma mater described him as "the man who has had the greatest impact on high school athletics in western Pennsylvania in the 100-year history." A native of Pittsburgh, Heberling attended Perry High School in the North Side neighborhood. He attended Washington & Jefferson College, graduating in 1949. There, he was a multi-sport athlete, letting three times in football, where he was a played running back alongside Melvin Bassi, Walter Cooper and “DeaconDan Towler, three times in baseball, where he was a top starting pitcher. During World War II, he served as a fighter pilot in the United States Navy, he worked as a teacher and coach of the football and basketball at East Washington High School.

He worked as a salesman for General Electric in Pittsburgh. During the 1970s, Heberling was a school board member for the North Allegheny School District. Heberling worked as a football official in high school and college football for 15 years and basketball for 25 years, he spent 23 years as an official in the National Football League, 16 years of which he spent as a crew chief. He spent another 14 years as an NFL observer, he was the referee for The Drive, one of the most famous events in professional football. He was an alternate on the officiating crew for Super Bowl XIII and Super Bowl XXI, he was the replay official for Super Bowl XXIII. In 1976, Heberling took over as executive director of the Western Pennsylvania Interscholastic Athletic League, which held supervisory control over scholastic sports in Western Pennsylvania. Under his leadership WPIAL grew from an organization being run from a basement into a well-respected and fiscally solvent sports organization, with a permanent headquarters, equality among the male and female sports, a lucrative contract bringing the WPIAL high school football championship to cable television.

He was a capable and headstrong executive, leading the Pittsburgh Post-Gazette to say that he "...took a hard stand on many WPIAL issues and ran the league with a certain boldness that infuriated some school officials and members of the media." In 1986, he secured the use of Three Rivers Stadium, Heinz Field, as the site of the WPIAL championships for all classes of WPIAL football. He retired from the WPIAL in June 1998 after 22 years. Heberling and his wife Jane had three sons and a daughter, he died at his home in McCandless, Pennsylvania on December 9, 2019 at the age of 94. Heberling was preceded in death by his wife of 65 years and his son Daniel, he is survived by two sons, a daughter, nine grandchildren and four great-grandchildren

1993 United States motorcycle Grand Prix

The 1993 United States motorcycle Grand Prix was the penultimate round of the 1993 Grand Prix motorcycle racing season. It took place on September 1993, at Laguna Seca. Everyone now knows Wayne Rainey’s career is over after his crash the previous round and that Kevin Schwantz is the 1993 world champion. Kenny Roberts on the question of retiring: “I’ve thought about it the last week, but Wayne wants to work in Grand Prix racing. That’s going to keep the edge sharp, because he wants to help the team, he wants to help riders, he wants to follow along in what I’ve done, and that’s going to keep me involved. Wayne’s a big, big part of my team, if he was disgusted with the whole thing yeah, it’d take a lot of the edge off, and he doesn’t show any signs of that and that’s going to keep me involved, otherwise I... well, you know, I can do anything I want to do, but he’s a driving force right now.”Schwantz on winning the championship: “Everybody’s thinking about Wayne, we all wish Wayne and Shae and Rex the best, but at the same time, I feel like we raced all season... to put ourselves in a position to be there to take advantage of any opportunities, any doors that were open.

I feel like we still earned it — we weren’t given it.”Rainey: “Kevin called. I said to him: You deserve it, and hey, I made the mistake. It doesn’t take anything away from what you did to win your championship.”John Kocinski takes the start from Mick Doohan and Alex Barros. Teammates Schwantz and Barros pass Kocinski. Barros starts to pull away, at Turn 11, Kocinski tries an inside pass on Schwantz that doesn't quite stick allows Doohan to come up and they go down the straight 3 abreast. Schwantz takes the hairpin ahead of Doohan and Kocinski. Doohan hunts down Barros and takes the lead. Kocinski passes Barros. Doohan's bike hops a bit on the exit of The Corkscrew and he loses his footing and rides it side-saddle but clips the hay bales on the left side of the track with his shoulder. He's hit by two bikes as he slides into the track, gets up but looks to be in pain. Kocinski's win is Cagiva’s first dry win in GP racing.

Saccharomyces bayanus

Saccharomyces bayanus is a yeast of the genus Saccharomyces, is used in winemaking and cider fermentation, to make distilled beverages. Saccharomyces bayanus like Saccharomyces pastorianus, is now accepted to be the result of multiple hybridisation events between three pure species, Saccharomyces uvarum, Saccharomyces cerevisiae and Saccharomyces eubayanus. Notably, most commercial yeast cultures sold as pure S. bayanus for wine making, e.g. Lalvin EC-1118 strain, have been found to contain S. cerevisiae cultures instead S. bayanus is used intensively in comparative genomics studies. Based on a computation-based experimental design system, Caudy et al. generated a rich resource for expression profiles for S. bayanus, used in several comparative studies in yeast systems, including expression patterns and nucleosome profiles. Yeast in winemaking Saccharomyces bayanus at ENTREZ genome project

Media Prima

Media Prima Berhad is Malaysian media and entertainment company with businesses in television, radio, out-of-home advertising, content creation and digital media. In television, the Group has four channels — TV3, NTV7, 8TV and TV9. Media Prima is the owner of The New Straits Times Press Berhad, Malaysia's largest publisher with three national news brands – New Straits Times, Berita Harian and Harian Metro, consumer brands; the Group ranks third in Malaysia in digital reach following the 2017 acquisition of REV Asia Holdings, one of Southeast Asia's leading digital media groups. In 2018, Media Prima became the number one choice for mobile content ahead of Facebook. Media Prima operates four free-to-air television stations. TV3 ntv7 8TV TV9 New Straits Times Berita Harian Harian MetroMedia Prima owns more than 98% equity interest in The New Straits Times Press Berhad, which owns three of Malaysia's most recognised print and online news brands – New Straits Times, BH and Harian Metro, as well as their respective weekend editions.

Fly FM Hot FM One FM Kool FM Ais Kacang SuperDealsRipple is an audience-focused digital media and commerce company. It includes four radio broadcast brands – Fly FM, Hot FM, One FM and Kool FM, a podcast platform – Ais Kacang, an e-commerce brand – SuperDeals, seven digital brands – Dhia, Lunaria, Likely and Wakeke. Big Tree Kurnia Outdoor UPD The Right Channel Gotcha Big Tree Seni Jaya Primeworks Studios Primeworks Distribution Grand Brilliance Monkey Bone The 8UnitMedia Prima's content creation arm, Primeworks Studios Sdn Bhd, is one of Malaysia's production houses with content output in diverse categories covering television and digital platforms. Media Prima Digital Media Prima Labs Rev Asia Holdings On 28 August 2018, Media Prima's Sri Pentas headquarters was forced to evacuate following a false bomb threat; the police gave an order to evacuate the company's office at 10.30 pm. About 200 Media Prima employees were evacuated from the building, they were allowed to re-enter the building after two hours of inspection.

The police did not find anything suspicious. Following the cancellation of Nightline, TV3 aired the announcement about the incident. PDRM's bomb detector unit arrived at 11.40 pm to handle the case. The Fire and Rescue Department received the phone call from the police at 11.19 pm following the incident. The case was investigated under Section 506 of the Penal Code and the police found it was a false bomb threat. Two men were arrested in Temerloh, Pahang on 29 August 2018