The Amboyna massacre was the 1623 torture and execution on Ambon Island of twenty-one men, including ten in the service of the English East India Company, as well as Japanese and Portuguese traders and a Portuguese man, by agents of the Dutch East India Company (VOC), on accusations of treason. It was the result of the intense rivalry between the East India companies of England and the United Provinces in the spice trade and remained a source of tension between the two nations until late in the 17th century.

Ambon Island is part of the Maluku Islands of Indonesia. The island has an area of 743.37 km2 (287.02 sq mi) and is mountainous, well watered, and fertile. Ambon Island consists of two territories: the city of Ambon to the south and various districts (kecamatan) of the Central Maluku Regency to the north. The main city and seaport is Ambon, which is also the capital of Maluku province, while those districts of Maluku Tengah Regency situated on Ambon Island had a 2020 Census population of 128,069. By mid 2023 those populations were estimated to have become 354,052 and 128,754 respectively, resulting in an all-island population of 482,806.