The 1899 South Australian colonial election was held on 29 April 1899 to elect all 54 seats in the South Australian House of Assembly. In the seat of Albert, the incumbent members were elected unopposed on 12 April, and the election in the seat of Northern Territory was held on 6 May. All 54 seats in the South Australian House of Assembly were up for election. The incumbent liberal government led by Premier of South Australia Charles Kingston in an informal coalition United Labor Party (ULP) led by Lee Batchelor defeated the conservative opposition led by Leader of the Opposition John Downer. Each district elected multiple members, with voters casting multiple votes. Although the conservatives won more seats, the liberal government retained power until later that year, when new conservative leader Vaiben Louis Solomon forced the government to resign, but only held office for one week. The liberals held government until the next election through leaders Frederick Holder and John Jenkins.

The premier of South Australia is the head of government in the state of South Australia, Australia. The Government of South Australia follows the Westminster system, with a Parliament of South Australia acting as the legislature. The premier is appointed by the governor of South Australia, and by modern convention holds office by virtue of their ability to command the support of a majority of members of the lower house of Parliament, the House of Assembly.