The 2nd Florida Infantry Regiment was an infantry regiment that fought in service of the Confederate States Army in the American Civil War.

Lewis Thornton Powell was an American Confederate soldier who attempted to assassinate William Henry Seward as part of the Lincoln assassination plot. Wounded at the Battle of Gettysburg, he later served in Mosby's Rangers before working with the Confederate Secret Service in Maryland. John Wilkes Booth recruited him into a plot to kidnap Lincoln and turn the president over to the Confederacy, but then decided to assassinate Lincoln, Seward, and Vice President Andrew Johnson instead, and assigned Powell the task to kill Seward.