AFI's 100 Years...100 Stars is the American Film Institute's list ranking the top 25 male and 25 female greatest screen legends of American film history and is the second list of the AFI 100 Years... series.

Katharine Houghton Hepburn was an American actress whose career as a Hollywood leading lady spanned six decades. She was known for her headstrong independence, spirited personality, and outspokenness, cultivating a screen persona that matched this public image, and regularly playing strong-willed, sophisticated women. She worked in a varied range of genres, from screwball comedy to literary drama, which earned her various accolades, including four Academy Awards for Best Actress—a record for any performer.