The Abbey of Our Lady of Gethsemani is a Catholic monastery in the United States near Bardstown, Kentucky, in Nelson County. The abbey is part of the Order of Cistercians of the Strict Observance, better known as the Trappists. Founded on December 21, 1848, and raised to an abbey in 1851, Gethsemani is considered to be the motherhouse of all Trappist and Trappistine monasteries in the United States. Gethsemani is the oldest Trappist monastery in the country that is still operating.

The Trappists, officially known as the Order of Cistercians of the Strict Observance and originally named the Order of Reformed Cistercians of Our Lady of La Trappe, are a Catholic religious order of cloistered monastics that branched off from the Cistercians. They follow the Rule of Saint Benedict and have communities of both monks and nuns that are known as Trappists and Trappistines, respectively. They are named after La Trappe Abbey, the monastery from which the movement and religious order originated. The movement first began with the reforms that Abbot Armand Jean le Bouthillier de Rancé introduced in 1664, later leading to the creation of Trappist congregations, and eventually the formal constitution as a separate religious order in 1892.