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Agarose gel electrophoresis
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Agarose gel electrophoresis is a method of gel electrophoresis used in biochemistry, molecular biology, genetics, and clinical chemistry to separate a mixed population of macromolecules such as DNA or
An agarose gel cast in tray, to be used for gel electrophoresis
An agarose gel cast in tray, to be used for gel electrophoresis
Gels of plasmid preparations usually show a major band of supercoiled DNA with other fainter bands in the same lane. Note that by convention DNA gel i
Gels of plasmid preparations usually show a major band of supercoiled DNA with other fainter bands in the same lane. Note that by convention DNA gel is displayed with smaller DNA fragments nearer to the bottom of the gel. This is because historically DNA gels were run vertically and the smaller DNA fragments move downwards faster.
Loading DNA samples into the wells of an agarose gel using a multi-channel pipette.
Loading DNA samples into the wells of an agarose gel using a multi-channel pipette.
Agarose gel slab in electrophoresis tank with bands of dyes indicating progress of the electrophoresis. The DNA moves towards anode.
Agarose gel slab in electrophoresis tank with bands of dyes indicating progress of the electrophoresis. The DNA moves towards anode.
Polymerase chain reaction
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The polymerase chain reaction is a laboratory method widely used to amplify copies of specific DNA sequences rapidly, to enable detailed study. PCR was invented in 1983 by American biochemist Kary Mul
Agarose gel electrophoresis
…from gel is to be used for further manipulation as the presence of contaminating sulfates may affect some subsequent procedures, such as ligation and PCR. Zero EEO agaroses however are undesirable for some applications as they may be made by adding positively charged groups and such groups can affect…
Placing a strip of eight PCR tubes into a thermal cycler
Placing a strip of eight PCR tubes into a thermal cycler
An older, three-temperature thermal cycler for PCR
An older, three-temperature thermal cycler for PCR
Ethidium bromide-stained PCR products after gel electrophoresis. Two sets of primers were used to amplify a target sequence from three different tissu
Ethidium bromide-stained PCR products after gel electrophoresis. Two sets of primers were used to amplify a target sequence from three different tissue samples. No amplification is present in sample #1; DNA bands in sample #2 and #3 indicate successful amplification of the target sequence. The gel also shows a positive control, and a DNA ladder containing DNA fragments of defined length for sizing the bands in the experimental PCRs.
DNA samples are often taken at crime scenes and analyzed by PCR.
DNA samples are often taken at crime scenes and analyzed by PCR.