The commune of Agen is the prefecture of the Lot-et-Garonne department in Nouvelle-Aquitaine in southwestern France. It lies on the river Garonne 135 kilometres southeast of Bordeaux; the city of Agen lies in the southern department Lot-et-Garonne in the Aquitaine region. The city centre lies on the east bank of the Garonne river close to the Canal de Garonne halfway between Bordeaux and Toulouse. Agen features an oceanic climate, in the Köppen climate classification. Winters feature cool to cold temperatures while summers are mild and warm. Rainfall is spread throughout the year; however, most sunshine hours are from March–September. From Occitan Agen, itself from Latin Aginnum, from a Celtic root agin- meaning "rock or height"; the town has a higher level of unemployment than the national average. Major employers include the pharmaceutical factory UPSA; the old centre of town contains a number of medieval buildings. The twelfth century Agen Cathedral, dedicated to Saint Caprasius, is one of the few large churches in France with a double nave, a regional trait found in the Church of the Jacobins in nearby Toulouse.

The Saint Hilaire church, dedicated to the theme of the Holy Trinity which the Saint in question did a lot to defend, is notable for its unusual statues in front of the Church – Moses on the right, St Peter on the left. The Fine Arts museum, Musée des Beaux Arts contains artefacts and sculptures from prehistoric times onwards; the art gallery contains several hundred works, including several by Goya, others by Bonnard and Seurat. The collection contains a large number of works by artists who lived locally; the museum is made up of twenty or so rooms. The Canal des Deux Mers, which joins the Mediterranean with the Atlantic, crosses the river Garonne at Agen via the town's famous canal bridge; the municipal theatre "Théâtre Ducourneau" presents theatre, classical concerts. The smaller "Théâtre du jour" has a resident theatre company presenting a variety of recent or older plays. There are two cinemas, one a commercial multiscreened affair, the other an arts cinema run by a voluntary organization.

The latter organizes film festivals every year. Rugby is popular in the town, the local team, SU Agen, is enthusiastically supported; the town serves as the base for the Team Lot-et-Garonne cycling team. The Gare d'Agen connects Agen with Bordeaux as well as Périgueux, it is around an hour around an hour from Bordeaux. The TGV train to Paris takes three hours and thirteen minutes with a stop in Bordeaux. Agen is connected, to both Toulouse and Bordeaux; the Agen Airport is serviced by Airlinair service to Paris Orly 6 days a week. It is used for business and leisure flying. Agen close to Bordeaux. Agen is the seat of a Roman Catholic diocese that comprises the Département of Garonne, it is a suffragan of the archdiocese of Bordeaux. Agen is twinned with: Tuapse, Russia Dinslaken, Germany Llanelli, United Kingdom Toledo, Spain Corpus Christi, United States As place of birthBernard Palissy, potter – according to some accounts, he may have been born in Saintes Joseph Justus Scaliger, scholar Pierre Dupuy, scholar Joseph Barsalou, physician Godefroi, comte d'Estrades and marshal Bernard Germain Étienne comte de La Ville-sur-Illon La Cépède, naturalist Jean Baptiste Bory de Saint-Vincent, naturalist Jacques Jasmin, Provençal poet Victor Rabu, architect who built many important churches in Montevideo, Uruguay Joseph Chaumié, politician William Grover-Williams racer and SOE agent Michel Serres and author Jacques Sadoul, author Jean Cruguet, jockey who won the U.

S. Triple Crown of thoroughbred racing. Alain Aspect, physicist Francis Cabrel, singer-songwriter and guitarist Bernard Campan and film director Emmanuel Flipo, artist Stéphane Rideau, actor Aymeric Laporte, footballerAs residenceNostradamus lived in Agen from 1531 until at least 1534, he was married to a local woman with. Agen is the "capital of the prune", a local product consumed as a sweet, either stuffed with prune purée or in pastries, or as a dessert, e.g. prunes soaked in Armagnac, a type of brandy. On the last weekend of August, a prune festival comprises rock concerts, circus performances and prune tastings; the first Jews settled in the town in the twelfth century AD. They were expelled from the town in 1306. A number of Jews returned to the town in 1315, a "Rue des Juifs" is documented since this period. In 1968, about 600 Jews lived in the town. A Jewish synagogue still exists in the town. SU Agen Lot-et-Garonne, a French rugby union club based in Agen Agenais, or Agenois, a former province of France INSEE statisticsNotes Site de la ville Office de tourisme Diocese of AgenCatholic Encyclopædia article

HHV capsid portal protein

HHV Capsid Portal Protein, or HSV-1 UL-6 protein, is the protein which forms a cylindrical portal in the capsid of Herpes simplex virus. The protein is referred to as the HSV-1 UL-6 protein because it is the transcription product of Herpes gene UL-6; the Herpes viral DNA exits the capsid via the capsid portal. The capsid portal is formed by twelve copies of portal protein arranged as a ring; each icosahedral capsid contains a single portal, located in one vertex. The portal is formed during initial capsid assembly and interacts with scaffolding proteins that construct the procapsid; when the capsid is nearly complete, the viral DNA enters the capsid by a mechanism involving the portal and a DNA-binding protein complex similar to bacteriophage terminase. Multiple studies suggest an evolutionary relationship between Capsid Portal Protein and bacteriophage portal proteins; when a virus infects a cell, it is necessary for the viral DNA to be released from the capsid. The Herpes virus DNA exits through the capsid portal.

The genetic sequence of HSV-1 gene UL-6 is conserved across the family Herpesviridae and this family of genes is known as the "Herpesvirus UL6-like" gene family. "UL-6" is nomenclature meaning that the protein is genetically encoded by the sixth open reading frame found in the viral genome segment named "Unique-Long". Research performed in 2004 used electron microscopy to predict that UL-6 forms 11, 12, 13, 14-unit polymers; the dodecameric form was found to be most likely. Refinements to the electron microscopy in 2007 allowed finding that the portal is a twelve -unit polymer present at one of the twelve capsid vertices instead of the UL-19 pentamer found at non-portal vertices. A study using deletion and mutation of the UL-6 amino acid sequence demonstrated the leucine residues in a predicted leucine zipper motif were required for formation of the dodecameric ring structure. Assembly of portal units is an initial step in constructing capsids of viral progeny. Capsids assembled in the absence of portals lack portals.

In 2003, gel eletrophoresis studies demonstrated that intact UL-6 portals associate in vitro with viral protein UL-26. This association is antagonized by that action of WAY-150138, a thiourea inhibitor of HHV encapsidation. Further investigation during 2006 showed that assembly of capsid with portal depends on interaction of UL-6 with "scaffolding" protein UL-26.5, amino acids 143 through 151. UL-6 associates with a UL-15/UL-28 protein complex during capsid assembly; the UL-15/UL-28 is believed to bind with viral DNA and serve the same purpose as terminase by packing viral DNA into the capsid during capsid assembly. The DNA exits the capsid in a single linear segment. DNA exit may be controlled by UL-6 and dependent on temperature or environmental proteins

Jim Copp and Ed Brown

Jim Copp and Ed Brown recorded and released nine albums of stories and songs for children between 1958 and 1971. Andrew James "Jim" Copp III wrote all of the stories and songs, played and recorded all of the music. Ed Brown designed and illustrated all of the duo's album covers. Both men performed the various characters' voices with the help of tape manipulation and were among the first to devise and use multi-track recording and electronic music for children's records. Copp and Brown's work has been compared to that of Lewis Carroll, Edward Lear, Dr. Seuss, Pee-wee Herman. Copp was born in Los Angeles and spent time in Alabama and Washington D. C.. His father was a prominent attorney. Instead the young James Copp had a penchant for the arts and grew up playing the piano and telling stories. At age 14 he was invited to play a Mozart concerto with the Los Angeles Philharmonic, he went on to study political science at Stanford University and creative writing as a graduate student at Harvard. After graduating, Copp entered a talent competition in Chicago and won a stint playing piano as a novelty performer for the Will Osborne Orchestra in 1939 began a career the following year as a cabaret piano comic in New York City under the name "James Copp the III and His Things".

As a solo performer, Copp caught the attention of Columbia Records talent scout John Hamond, who booked him on bills with Teddy Wilson, Lena Horne, Art Tatum, Billie Holiday and others at the Café Society. In 1941 Copp made his first album, James Copp 3, a three-disc 78 RPM folio recorded at Reeves Sound Studios and released by Liberty Music Shops in New York City; the album's six tracks featured Copp's piano-playing and comedic nonsense storytelling, adapted from his nightclub act, the album's jacket pictured a photo of Copp's upper body superimposed over a collection of Copp's own doodles. Many of Copp's early bits riff off of children's nursery rhymes, like "Mary Had a Little Lamb", "Twinkle Twinkle Little Star" and "Fuzzy-Wuzzy was a Bear", but intermingled with expletives and references to graphic violence. In 1941, one of Copp's comedy narratives was performed by comic Doodles Weaver for a Soundie movie short, "Arabella and the Water Tank." Copp and Weaver would work on comedy scripts for radio and club routines off and on for the next several years, until Doodles moved to California in 1946, to join Spike Jones and his City Slickers.

Evidently none of this material was performed publicly. Copp's show business career was interrupted by World War II when he was shipped off to Europe in 1942, he commanded an intelligence unit for the Normandy Invasion. After the war he returned to work in New York, but grew tired of working the nightclub circuit, so moved back to Los Angeles where he wrote and illustrated the society column "Skylarking with James Copp" for the Los Angeles Times. During the 1950s Copp reworked some of his nightclub routines for a younger audience and recorded them on a wire recorder, he sent sample recordings to Capitol Records. Capitol's executives liked the material, but wanted them to be performed by Jerry Lewis because he was a celebrity and would therefore sell more records. Copp agreed to let them have one story called'"The Noisy Eater", which Capitol had Lewis record and the company issued it on 78 and 45 rpm records in 1955. After the record achieved moderate success, Capitol wanted to buy the rest of Jim Copp's recordings, but having been paid little for "The Noisy Eater", Copp refused to sell his material to them.

Instead he decided to have a go at producing his own material, the wire-recorded demos he'd sent to Capitol ended up as the material for his first long-playing children's album. Jim Copp met the artist Ed Brown at a society party in Los Angeles and the two men began a lifelong friendship. Brown, a graduate of the University of South Carolina, was a linguist and graphic designer, Copp shared with him an idea for a children's album that would incorporate Copp's own numerous musical talents with Brown's talent for design. For this first record, Copp played all of the instruments, performed all of the voices and contributed some of the artwork while Brown handled the album's overall design and marketing; the album Jim Copp Tales featured a picture wheel that listeners could turn to see Copp's own doodles that illustrated each story. Copp used the nickname "Jim" instead of his cabaret name, in case the project fell flat, but the album did well; the record received wide exposure on radio and TV, as well as bids for exclusive distribution by a department store chains I.

Magnin, Neiman-Marcus, Bloomingdale's and FAO Schwarz. Copp and Brown soon traveled the U. S. touring to retail outlets with their luggage stuffed with records. After the success of Jim Copp Tales and Brown engaged in a laborious routine of annually self-producing albums and releasing them on their own label Playhouse Records. A record's creation began with Copp sitting at home writing songs and stories, while Ed Brown worked on the jacket design at his own house. Copp and Brown would record all of the sound effects, speeches and stories in segments in multiple takes until satisfied with the results. Copp recorded instruments in different rooms at his parents' house: voices were taped in the kitchen, the piano in the living room, the celeste in a bedroom, the pump organ in the bathroom, sound effects in the bathtub; the entire recording was done with one microphone and three monaural Ampex tape recorders, with which Copp devised his own overdubbing technique by ping-ponging between the tape decks to build up layers of sound.

This recording method allowed the duo to create whole classrooms and housefuls of different voices—as many as 90 on a single tra