The ancient universities of Scotland are medieval and renaissance universities that continue to exist in the present day. Together, the four universities are the oldest universities in continuous operation in the English-speaking world after the universities of Oxford and Cambridge. The majority of the ancient universities of the British Isles are located within Scotland, and have a number of distinctive features in common, being governed by a series of measures laid down in the Universities (Scotland) Acts 1858–1966. The Universities (Scotland) Act 1966 uses the term 'older universities' to refer to St Andrews, Glasgow, Aberdeen and Edinburgh. The four universities are generally regarded as the country's most selective, eminent and well-ranked universities.

A medieval university was a corporation organized during the Middle Ages for the purposes of higher education. The first Western European institutions generally considered to be universities were established in present-day Italy, including the Kingdoms of Sicily and Naples, and the Kingdoms of England, France, Spain, Portugal, and Scotland between the 11th and 15th centuries for the study of the arts and the higher disciplines of theology, law, and medicine. These universities evolved from much older Christian cathedral schools and monastic schools, and it is difficult to define the exact date when they became true universities, though the lists of studia generalia for higher education in Europe held by the Vatican are a useful guide.