Anstruther is a coastal town in Fife, Scotland, situated on the north-shore of the Firth of Forth and 9 mi (14 km) south-southeast of St Andrews. The town comprises two settlements, Anstruther Easter and Anstruther Wester, which are divided by a stream, the Dreel Burn. With a population of 3,500, it is the largest community on the Firth of Forth's north-shore coastline known as the East Neuk. To the east, it merges with the village of Cellardyke.

Fife is a council area, historic county, registration county and lieutenancy area of Scotland. It is situated between the Firth of Tay and the Firth of Forth, with inland boundaries with Perth and Kinross and Clackmannanshire. By custom it is widely held to have been one of the major Pictish kingdoms, known as Fib, and is still commonly known as the Kingdom of Fife within Scotland. A person from Fife is known as a Fifer. In older documents the county was very occasionally known by the anglicisation Fifeshire.