The aphotic zone is the portion of a lake or ocean where there is little or no sunlight. It is formally defined as the depths beyond which less than 1 percent of sunlight penetrates. Above the aphotic zone is the photic zone, which consists of the euphotic zone and the disphotic zone. The euphotic zone is the layer of water in which there is enough light for net photosynthesis to occur. The disphotic zone, also known as the twilight zone, is the layer of water with enough light for predators to see but not enough for the rate of photosynthesis to be greater than the rate of respiration.

The vampire squid is a small cephalopod found throughout temperate and tropical oceans in extreme deep sea conditions. The vampire squid uses its bioluminescent organs and its unique oxygen metabolism to thrive in the parts of the ocean with the lowest concentrations of oxygen. It has two long retractile filaments, located between the first two pairs of arms on its dorsal side, which distinguish it from both octopuses and squids, and places it in its own order, Vampyromorphida, although its closest relatives are octopods. As a phylogenetic relict, it is the only known surviving member of its order.