Laguna de Apoyo Nature Reserve is a nature reserve located between the departments of Masaya and Granada in Nicaragua. Lake Apoyo was declared a nature reserve in 1991 and is managed by the Ministry of the Environment and Natural Resources (MARENA) and comprises one of 78 protected areas of Nicaragua. Activities within the Laguna de Apoyo Nature Reserve are regulated by its management plan, approved in 2010, which prohibits the construction of housing within the reserve and use of motorized vehicles on the lake. Geological data suggests that Lake Apoyo originated about 23,000 years ago.

The protected areas of Nicaragua are areas that have natural beauty or significance and are protected by Nicaragua. Nicaragua has 78 protected areas that cover 22,422 km2, about 17.3% of the nations landmass. The National System of Protected Areas (SINAP) is administered by the Ministry of the Environment and Natural Resources (MARENA).