The Battle of Nineveh is conventionally dated between 613 and 611 BC, with 612 BC being the most supported date. Rebelling against the Assyrians, an allied army which combined the forces of Medes and the Babylonians besieged Nineveh and sacked 750 hectares of what was, at that time, one of the greatest cities in the world. The fall of Nineveh led to the destruction of the Neo-Assyrian Empire over the next three years as the dominant state in the Ancient Near East. Archeological records show that the capital of the once mighty Assyrian Empire was extensively de-urbanized and depopulated in the decades and centuries following the battle. A garbled account of the fall of the city later led to the story of the legendary king Sardanapalus.

Nabopolassar was the founder and first king of the Neo-Babylonian Empire, ruling from his coronation as king of Babylon in 626 BC to his death in 605 BC. Though initially only aimed at restoring and securing the independence of Babylonia, Nabopolassar's uprising against the Neo-Assyrian Empire, which had ruled Babylonia for more than a century, eventually led to the complete destruction of the Assyrian Empire and the rise of the Neo-Babylonian Empire in its place.