Brownstown is a borough in Cambria County, United States. The population was 744 at the 2010 census, it is part of the Johnstown, Pennsylvania Metropolitan Statistical Area and part of Johnstown's urban area. Brownstown is located in southwestern Cambria County at 40°20′01″N 78°56′14″W, it is bordered to the south by Westmont. The borough sits on a hill on the west side of the Conemaugh River valley and is 2 miles northwest of the center of Johnstown by road. According to the United States Census Bureau, the borough has a total area of 0.23 square miles, all of it land. As of the census of 2000, there were 883 people, 354 households, 259 families living in the borough; the population density was 3,467.6 people per square mile. There were 372 housing units at an average density of 1,460.9/sq mi. The racial makeup of the borough was 99.32% White, 0.23% African American, 0.11% Asian, 0.34% from two or more races. There were 354 households, out of which 31.1% had children under the age of 18 living with them, 56.8% were married couples living together, 9.6% had a female householder with no husband present, 26.6% were non-families.
23.4% of all households were made up of individuals, 13.6% had someone living alone, 65 years of age or older. The average household size was 2.49 and the average family size was 2.94. In the borough the population was spread out, with 21.5% under the age of 18, 7.0% from 18 to 24, 27.5% from 25 to 44, 25.4% from 45 to 64, 18.6% who were 65 years of age or older. The median age was 41 years. For every 100 females there were 92.4 males. For every 100 females age 18 and over, there were 88.8 males. The median income for a household in the borough was $33,043, the median income for a family was $36,645. Males had a median income of $25,000 versus $17,386 for females; the per capita income for the borough was $13,494. About 5.3% of families and 8.0% of the population were below the poverty line, including 8.3% of those under the age of 18 and 5.2% who were 65 or older
Carlos Zeferino Torreblanca Galindo is a Mexican politician affiliated with the PRD Party of the Democratic Revolution now affiliated with the PT and former Governor of Guerrero. He is the first non PRI member to hold the position. Zeferino Torreblanca is the son of Luisa Galindo Ochoa, he holds a bachelor's degree in accounting from the Monterrey Institute of Technology and Higher Education. In 1993 he unsuccessfully ran for municipal president of Acapulco representing the PRD. In 1994, he secured a seat in the Chamber of Deputies of Mexico via proportional representation to serve during the LVI Legislature. In 1999 he won. In February 2005 he was elected Governor of Guerrero and left office in March 31, 2011. In 2015 he received the backing of the PAN and ran again for municipal president of Acapulco, but lost. In 2018 he is running again for this position, but this time with the PT; such realignments are common in present-day Mexican politics. List of mayors of Acapulco
Jenna is an album by Gerald Wilson's Orchestra of the 90's recorded in 1989 and released on the Discovery label. AllMusic rated the album with 4½ stars. All compositions by Gerald Wilson except. "Love for Sale" - 4:13 "Jenna" - 9:46 "Carlos" - 5:19 "Back to the Roots" - 5:01 "The Wailer" - 4:28 "Blues for Yna Yna" - 6:53 "B-Bop & the Song" - 5:19 "Couldn't Love, Couldn't Cry" - 2:34 "Yarddog Mazurka" - 3:14 "48 Years Later" - 3:20 "Lunceford Special" - 3:33 "Margie" - 3:12 "Flying Home" - 3:23 Gerald Wilson - arranger, conductor Rick Baptist, Ronald Barrows, Oscar Brashear, Robert Clark, Snooky Young - trumpet Luis Bonilla, Thurman Green, Charles Loper - trombone Maurice Spears - bass trombone Daniel House, Carl Randall, John Stephens, Louis Taylor Jr. - saxophones Randall Willis - baritone saxophone, alto saxophone Michael Cain - piano Anthony Wilson - guitar Stanley Gilbert - bass Mel Lee - drums
ClickSeq is a click-chemistry based method for generating Next-Generation Sequencing libraries for deep-sequencing platforms including Illumina, HiSeq, MiSeq and NextSeq. Its function is similar to most other techniques for generating RNAseq or DNAseq libraries in that it aims to generate random fragments of biological samples of RNA or DNA and append specific sequencing adaptors to either end of every fragment, as per the requirements of the particular sequencing platform to be used. In ClickSeq, reverse transcription reactions are supplemented with small amounts of 3’-azido-nucleotides at defined ratios to deoxyribonucleotides. AzNTPs are chain-terminators and therefore induce the stochastic termination of cDNA synthesis at an average length determined by the ratio of AzNTPs to dNTPs; this results in the production of single-stranded cDNA fragments that contain an azido-group at their 3' ends. These 3'-azido-blocked cDNA molecules are purified away from the components of the RT reaction, subsequently'click-ligated' to 5’ alkyne-modified DNA adaptors via copper-catalysed azide-alkyne cycloaddition.
This generates ssDNA molecules with unnatural triazole-linked DNA backbones. These templates are used in PCR reactions and amplified to generate a cDNA sequencing library with the appropriate 5' and 3' sequencing adapters and indices required for Next-Generation Sequencing. ClickSeq has predominantly been used to sequence viral RNA genomes such as Flock House virus, Cricket Paralysis virus, Zika virus, due to its resilience to artifactual chimera formation. Poly-ClickSeq is a variant of ClickSeq designed to target the junction of the 3' Untranslated Regions and poly-tails of the messenger RNAs of higher-order organisms and of RNA viruses infecting these cells types; the core principle is similar to ClickSeq, the reverse-transcription step uses an oligo-dT primer to initiate cDNA synthesis from within the poly tail and only three 3'azido-nucleotides are supplemented. Due to the omission of AzTTP, stochastic termination of cDNA synthesis cannot occur during reverse transcription of the poly-tail.
Rather, termination can only occur in the 3'UTR at a distance upstream of the poly tail defined by the ratio of AzVTPs to dNTPs. ClickSeq and Poly-ClickSeq provide specific applications over other common RNA-seq techniques; these include: Removal of RNA fragmentation steps: When the reverse-transcription step is random-primed and cDNA synthesis is terminated by the 3'-azido-nucleotides, cDNA fragments can be generated without chemical, mechanical or enzymatic fragmentation of the sample RNA Removal of RNA/DNA ligase enzymes: In ClickSeq, there are no RNA or DNA ligation steps, as are required in most Next-Generation Sequencing library synthesis strategies Reduction of artifactual recombination: In the original ClickSeq publication, Routh et al. demonstrated that the artifactual generation of cDNA chimeras was reduced when using ClickSeq. This allowed the authors to detect rare RNA recombination events that arise during the replication of Flock House virus. Poly-ClickSeq does not require enrichment or purification of mRNA or viral RNAs from biological specimens.
Rather, Poly-ClickSeq can be performed in a simple manner directly from crude RNA or total cellular RNA extracted from biological specimens. The copper-catalyst required for CuAAC may induce oxidative damage of the template DNA
Jonathan Dunn-Rankin was an American actor, television journalist and gay activist. Jonathan Dunn-Rankin was born on November 1930 in New Jersey, he graduated from Rollins College after serving in the US Army in France, he earned a master's degree from the Columbia University Graduate School of Journalism in 1958. Dunn-Rankin first interned at CBS News in New York City, he worked as a television journalist in Florida, Cleveland and Phoenix, Arizona. From 1965 to 1977, he worked for a television station based in San Diego. Dunn-Rankin became a stage actor at the Old Globe Theatre in San Diego. Dunn-Rankin was "a founder of the San Diego Democratic Club, a leader of the Gay Academic Union and an early supporter of Lambda Archives", he was the chairman of the Diversionary Theatre, an LGBT theater in San Diego. Dunn-Rankin had a partner, David Ramos, resided in San Diego, he died on December 12, 2014. Jonathan Dunn-Rankin on IMDb
Berserker is the third album released by Jane, composed of Animal Collective member Panda Bear and Scott Mou. Berserker is Jane's first release on Paw Tracks; the songs consist of Mou sampling other musicians' backing tracks. According to Lennox, "It’s like a mix CD with toasting over the top. We make the songs our own because the way we move from track to track is unique.". Lennox described the writing process on the Paw Tracks website: "Scotty and I worked together at a record store in NYC, he was a DJ around town and he still is I think. We both liked dance music and dance music from the beginning and I mean stomps and shouts and claps and stuff like that. Of course we like all kinds of other stuff too. We played once at the Animal Collective practice space, but found it much more pleasant to play at Scotty's home in Greenpoint where he had his mixer and simple microphones and we would drink brews and talk about all kinds of things and play. I would sing about stuff I was thinking about that day and Scotty would move with it, playing jams and it would all kind of pour out.
We liked all the mechanical robo dance jams from Detroit and Chicago and Germany but we wanted to do something with less 0's and 1's and more souls. It was about hanging out together and talking and playing music, about talking together and hanging out and thinking and feeling and having fun and dancing most of all."