The Castro of Vila Nova de São Pedro is a Chalcolithic archaeological site in the civil parish of Vila Nova de São Pedro, municipality of Azambuja, in the Portuguese Estremadura area of Lezíria do Tejo. It is important for the discovery of thousands of arrowheads within its fortified settlement, associated with the Chalcolithic period of human settlement. associated with the long-lived fortified town, or castro, of Zambujal, near the municipality of Torres Vedras. The period of "urban" settlement lasted from 2600 to 1300 BCE, and was a contemporary of the southeastern Spanish settlements of Los Millares and El Argar.

The Chalcolithic was an archaeological period characterized by the increasing use of smelted copper. It followed the Neolithic and preceded the Bronze Age. It occurred at different periods in different areas, but was absent in some parts of the world, such as Russia, where there was no well-defined Copper Age between the Stone and Bronze ages. Stone tools were still predominantly used during this period.