Cincinnati Children's Hospital Medical Center (CCHMC) is an academic pediatric acute care children's hospital located in the Pill Hill neighborhood of Cincinnati, Ohio. The hospital has 652 pediatric beds and is affiliated with the University of Cincinnati Academic Health Center. The hospital provides comprehensive pediatric specialties and subspecialties to pediatric patients aged 0–21 throughout southern Ohio and northern Kentucky, as well as patients from around the United States and the world. Cincinnati Children's Hospital Medical Center also treats adults, including adults with congenital heart disease and young adults with blood disease or cancer. Cincinnati Children's Hospital Medical Center also features a Level 1 Pediatric Trauma Center, 1 of 4 in the state. Cincinnati Children's is home to a large neonatology department that oversees newborn nurseries at local hospitals around Ohio. The hospital features an AAP verified 89-bed Level IV (highest possible) Newborn Intensive Care Unit.

Nationwide Children's Hospital is a nationally ranked pediatric acute care teaching hospital located in the Southern Orchards neighborhood of Columbus, Ohio. The hospital has 673 pediatric beds and is affiliated with the Ohio State University College of Medicine. The hospital provides comprehensive pediatric specialties and subspecialties to infants, children, teens, and young adults aged 0–21 throughout Ohio and surrounding regions. Nationwide Children's Hospital also sometimes treats adults that require pediatric care. Nationwide Children's Hospital also features an ACS-verified Level 1 Pediatric Trauma Center, one of four in the state. The hospital has affiliations with the nearby Ohio State University Wexner Medical Center. Nationwide Children's Hospital is located on its own campus and has more than 1,379 medical staff members and over 11,909 total employees.