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DNA profiling

DNA profiling is the process of determining an individual's DNA characteristics. DNA analysis intended to identify. DNA profiling is a forensic technique in criminal investigations, comparing criminal suspects' profiles to DNA evidence so as to assess the likelihood of their involvement in the crime, it is used in parentage testing, to establish immigration eligibility, in genealogical and medical research. DNA profiling has been used in the study of animal and plant populations in the fields of zoology and agriculture. Starting in the 1980s scientific advances allowed for the use of DNA as a material for the identification of an individual; the first patent covering the direct use of DNA variation for forensics was filed by Dr. Jeffrey Glassberg in 1983, based upon work he had done while at Rockefeller University in 1981. In the United Kingdom, Geneticist Sir Alec Jeffreys independently developed a DNA profiling process in beginning in late 1984 while working in the Department of Genetics at the University of Leicester.

The process, developed by Jeffreys in conjunction with Peter Gill and Dave Werrett of the Forensic Science Service, was first used forensically in the solving of the murder of two teenagers, raped and murdered in Narborough, Leicestershire in 1983 and 1986. In the murder inquiry, led by Detective David Baker, the DNA contained within blood samples obtained voluntarily from around 5,000 local men who willingly assisted Leicestershire Constabulary with the investigation, resulted in the exoneration of a man who had confessed to one of the crimes, the subsequent conviction of Colin Pitchfork. Pitchfork, a local bakery employee, had coerced his coworker Ian Kelly to stand in for him when providing a blood sample—Kelly used a forged passport to impersonate Pitchfork. Another coworker reported the deception to the police. Pitchfork was arrested, his blood was sent to Jeffrey's lab for processing and profile development. Pitchfork's profile matched that of DNA left by the murderer which confirmed Pitchfork's presence at both crime scenes.

Although 99.9% of human DNA sequences are the same in every person, enough of the DNA is different that it is possible to distinguish one individual from another, unless they are monozygotic twins. DNA profiling uses repetitive sequences that are variable, called variable number tandem repeats, in particular short tandem repeats known as microsatellites, minisatellites. VNTR loci are similar between related individuals, but are so variable that unrelated individuals are unlikely to have the same VNTRs. In India DNA fingerprinting was started by Dr. Lalji Singh. Singh was an Indian scientist who worked in the field of DNA fingerprinting technology in India, where he was popularly known as the "Father of Indian DNA fingerprinting"; the process, developed by Glassberg and independently by Jeffreys, begins with a sample of an individual's DNA. Reference samples are collected through a buccal swab; when this is unavailable other methods may be needed to collect a sample of blood, semen, vaginal lubrication, or other fluid or tissue from personal use items or from stored samples.

Samples obtained from blood relatives can indicate an individual's profile, as could previous profiled human remains. A reference sample is analyzed to create the individual's DNA profile using one of the techniques discussed below; the DNA profile is compared against another sample to determine whether there is a genetic match. When a sample such as blood or saliva is obtained, the DNA is only a small part of what is present in the sample. Before the DNA can be analyzed, it must be purified. There are many ways this can be accomplished; the cell and nuclear membranes need to be broken up to allow the DNA to be free in solution. Once the DNA is free, it can be separated from all other cellular components. After the DNA has been separated in solution, the remaining cellular debris can be removed from the solution and discarded, leaving only DNA; the most common methods of DNA extraction include organic extraction, Chelex extraction, solid phase extraction. Differential extraction is a modified version of extraction in which DNA from two different types of cells can be separated from each other before being purified from the solution.

Each method of extraction works well in the laboratory, but analysts selects their preferred method based on factors such as the cost, the time involved, the quantity of DNA yielded, the quality of DNA yielded. After the DNA is extracted from the sample, it can be analyzed, whether it is by RFLP analysis or quantification and PCR analysis; the first methods for finding out genetics used for DNA profiling involved RFLP analysis. DNA is cut into small pieces using a restriction enzyme; this generates DNA fragments of differing sizes as a consequence of variations between DNA sequences of different individuals. The fragments are separated on the basis of size using gel electrophoresis; the separated fragments are transferred to a nitrocellulose or nylon filter. The DNA fragments within the blot are permanently fixed to the filter, the DNA strands are denatured. Radiolabeled probe molecules are added that are complementary to sequences in the gen

Wang Jianzhao

Wang Jianzhao was a warlord late in the Chinese dynasty Tang Dynasty, who controlled Jingnan Circuit from 887 to 888, Wutai Circuit from 888 to 896, when he surrendered to Wang Jian. Little is known about Wang Jianzhao's background, as there was no biography for him in any of the official histories; the first historical reference to him was in 887, as of which time he was serving under Zhao Deyin, who had claimed the title of acting military governor of Shannan East Circuit under allegiance to Qin Zongquan —, a Tang general but who had rebelled against the rule of Emperor Xizong and claimed the title of emperor himself at Cai Prefecture. Late in 887, Zhao attacked Tang's Jingnan Circuit and killed its military governor Zhang Gui, he left Wang in charge of Jingnan, whose capital Jiangling Municipality was said to have only a few hundred families left after the warfare. In 888, the Tang prefect of Gui Prefecture, Guo Yu attacked expelled Wang. Wang fled to Qian Prefecture; some time perhaps around 890, Wang submitted to Tang imperial authority and was made the military governor of Wutai Circuit.

Little is known about Wang Jianzhao's governance of Wutai. In 896, Guo Yu, along with his subordinate Xu Cun, headed west on the Yangtze River to capture Wang's territory. Wang was unable to resist their attack, he abandoned Qian Prefecture and withdrew to Fengdu. Cheng made his subordinate Zhao Wu the acting military governor of Wutai. Zhao thereafter made repeated attacks on Wang at Fengdu. Wang could not resist Zhao's attacks, so he surrendered to Wang Jian the military governor of Xichuan Circuit; that was the last historical reference to Wang Jianzhao, it is not known whether he served in any campaigns under Wang Jian or when he died

Lynk & Co 03

Lynk & Co 03 is a compact sedan manufactured by Chinese-Swedish automaker Lynk & Co as the sedan version of Lynk & Co 02. The Lynk & Co 03 sedan went on sale in October 2018; the Lynk & Co 03 went on sale on October 2018 and was designed mechanically similar to Lynk & Co 02, based on the Compact Modular Architecture platform. Lynk & Co 03 made its first appearance in September 2018 during the Chengdu Auto Show in China; the new car provides engine options including a 156 hp 1.5 liter turbo, a 180 hp 1.5 liter turbo, a 190 hp 2.0 liter turbo, gearbox available are a 6-speed manual transmission and a 7-speed dual-clutch transmission. It sells between 116,800-151,800 RMB in China; the 03 is set to take part in the 2019 World Touring Car Cup with the car being build by Cyan Racing according to the TCR regulations. Thed Björk, Yann Ehrlacher, Yvan Muller and Andy Priaulx are scheduled to compete with the 03 TCR

I Am Robot and Proud

Shaw-Han Liem, better known by the alias I am Robot and Proud, is a Canadian indie electronic musician and video game designer from Toronto, Ontario. He has released albums under the labels Darla and & Records, he co-created PS Vita game Sound Shapes. Liem was raised in Toronto, Ontario. Classically trained as a pianist, Liem taught himself how to play guitar and began creating electronic music under the name I Am Robot and Proud in 2000 while completing a degree in computer science, he has been a member of Sea Snakes and Tusks, has worked with Jim Guthrie and Nathan Lawr. While Liem writes and records on his own, 2015's Lights and Waves is the first album conceived and recorded with Liem's live band; the recording was prompted by the composing of band-focused arrangements of his previous work. The I am Robot and Proud live band consists of Toronto musicians Robin Buckley, Mike Smith and Jordan Howard. Previous members have included Jim Guthrie, Samir Khan, Gus Weinkauf, Simon Osbourne and Jeremy Strachan.

In 2007, he participated in the global launch events for Tenori-On, an instrument designed by Toshio Iwai. In 2009, Liem collaborated on a performance with media artist Daito Manabe, combining Manabe's physical music visualizations with improvised composition. In 2010 he performed a live collaboration set with Boredoms drummer Senju Muneomi and Tokyo computer-composer Yuri Miyauchi. Liem collaborated with game designer Jonathan Mak on PlayStation 3 and PS Vita game Sound Shapes, serving as a co-designer and composer, he participated in a roundtable discussion on the topic of independent videogame music at the 2009 Game Developers Conference together with the composers of independent game titles PixelJunk Eden and Night Game. Sound Shapes won the 2012 Game Critics Awards for best mobile/handheld game, was declared E3 Game of Show by It won Best Song and Best Mobile game at the Spike Video Game Awards; the Catch You Make Me This Happy Grace Days The Electricity in Your House Wants to Sing Catch/Spring Summer Autumn Winter Uphill City Uphill City Remixes & Collaborations Touch/Tone People Music Light and Waves Lucky Static Spring Summer Autumn Winter Electric Avenue 6 My Sky Your Sky Logika / Paññā Little Keys on Anything For Progress One, Three, Five, Seven on Songs of Seven Colors Jean Meance on Habitat Lasah collaboration track "WELCOME TO THE MABOROSHI CLUB" on the album Mother Qrion remix track "Mizu" on the album Sink Lasah collaboration track "Give Up" on the album I Love You Akira Kosemura remix track "虹の彼方" on 虹の彼方 single The Girl and The Robots remix track "單人探戈" on 平行宇宙 Shintaro Aoki collaboration track "Two Pianos" on Mizukage Compilation 16 Baiyon collaboration track "Madder Red" on Vibes Against Vibes Minotaurs Pink Floyd remix on The Thing Remixed Yomoya Kitaineiro remix on Yawn Autumnleaf One Day remix on The Foot Remix EP Pupa Dreaming Pupa remix on Remix EP Jim Guthrie All Gone alternate mix on Now More Than Ever LP Yuri Miyauchi _ael remix on Toparch Brian Alfred It's Already The End of The World Installation/DVD Soundtrack Deastro Green and Nordics on Tone Adventure #3 no.9 Princess on Usual Revolution & Nine Mitsuki Aira Happiness Land on Barbie Barbie Gentleman Reg We're in a Thunderstorm on Jet Black De De Mouse Sundown River on Sunset Girls

Yalgoo, Western Australia

Yalgoo is a town in the Murchison region, 499 kilometres north-north-east of Perth and 118 kilometres east-north-east of Mullewa. Yalgoo is in the local government area of the Shire of Yalgoo. Before it was settled as a town the Yalgoo area was used as grazing land for European settlers including the Morrissey and Broad families. Flocks of sheep were herded onto the rich pastures during the wet growing season and driven back to coastal properties for shearing before summer. Over time the graziers saw the value in the Yalgoo land and began to establish the first sheep stations. Gold was discovered in the area in the early 1890s, by 1895 there were 120 men working the diggings and buildings being erected; the goldfield warden asked for a townsite to be surveyed and gazetted, following survey the townsite of Yalgu was gazetted in January 1896. It was once the location of an important railway station on the Northern Railway. Yalgoo's importance declined in the years after World War II after the forging of an all-weather road between Wubin and Paynes Find, across Lake Moore.

In early 1898 the population of the town was 500 males and 150 females. In 1921-22 the priest-architect and parish priest of Yalgoo, Mgr John Hawes and built the Dominican convent school and chapel of St Hyacinth: Yalgoo children attended the school until it was closed for lack of pupils in 1950; the timber framed school building was removed. The derelict chapel was restored and re-opened in 1981. Erection of a state battery was completed in October of the same year. Yalgoo is an Aboriginal name first recorded for Yalgoo Peak by the surveyor John Forrest in 1876; the name is said to mean "blood" or "place of blood", derived from the word "Yalguru". An alternative view is that it is derived from the Yalguru bush which abounds in the area, has blood red sap; the spelling Yalgu was used because of spelling rules for Aboriginal names adopted by the Lands & Surveys Department. Within a month the Lands & Surveys had decided reluctantly to use the original Yalgoo spelling, this spelling has been used since.

Some doubt about the spelling being changed resulted in an amendment from Yalgu to Yalgoo being gazetted in 1938. Yalgoo has a hot desert climate with mild winters; the name Yalgoo is used as a name for a crater on the planet Mars, without commemorating the town. Yalgoo - the ecological region Thundelarra Shire of Yalgoo - the local government region Northern Railway December 2010 Gascoyne River flood Western Australian Land Information Authority. "History of country town names – Y". Retrieved 25 May 2007. Palmer, Alex. Yalgoo Fremantle, W. A: Lap Industries. ISBN 0-9590584-0-0


Labriformes is an order of ray-finned fishes which includes the wrasse and parrotfishes, within the clade Percomorpha. Some authors include the Labroformes as the clade Labroidei within the Perciformes while others include more families within the Labriformes, such as the cichlids and damselfishes, but the 5th edition of Fishes of the World includes just three listed in the section below and includes 87 genera and about 630 species; the following three families are classified within the Labriformes: Labridae Cuvier, 1816 Odacidae Günther 1861 Scaridae Rafinesque, 1810